Confining Electrochemistry to Nanopores by Yi-Tao Long;

Author:Yi-Tao Long; [Long, Yi-Tao]
Language: eng
Format: epub
ISBN: 9781839160905
Publisher: Book Network Int'l Limited trading as NBN International (NBNi)
Published: 2020-10-13T00:00:00+00:00

Figure 4.7 Real-time monitoring the DNA duplex unzipping process with the characteristic current signals. (a) Top: a current signature of a type I event. Bottom: diagrams for the molecular mechanism of the DNA duplex unzipping and translocation processes. (b) Histogram of the duration time of level 1, which refers to the unzipping time. The histogram is fitted to an exponential function. (c) Histograms of current blockade (ΔI/I0) of level 1, level 2 and level 3, respectively. The histograms of ΔI/I0 are fitted to the Gaussian function. (d) Relationship between the duration time of level 1 and the applied voltage for the unzipping of DNA duplexes. Inset: relationship between the duration time of level 3 and the applied voltage. Reproduced from ref. 31 with permission from the Royal Society of Chemistry.

More recently, solid-state nanopores with small diameter fabricated by CDB were also used for the detection of human telomere repeat sequences (Figure 4.8a).23 The ultra-low current detection system in this study employed an electric circuit, which could achieve solid-state nanopore fabrication and single molecule detection, simultaneously (Figure 4.8b). The telomere repeat sequence was used as a probe to determinate the confinement effect in the nanopore analysis. The results showed that the nanopore with comparable volume of analytes exhibited an excellent capability for single molecule sensing. For example, the translocation of telomeric DNA through the confined nanopore with a diameter of 1.7 nm generated distinct current blockades with long duration time, which reflected the conformational changes of the DNA molecules (Figure 4.8c). However, the translocation events for the human telomeric DNA through a larger nanopore with a dimeter of 6.2 nm generated short duration time (Figure 4.8d). It showed that the remarkable electrochemical confinement effect would enhance the sensing performance of nanopore platform, and the unique nanopore confinement could be further used for the accurate analysis of short-lived metastable conformations of DNAs or peptides.

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